Figure 6. Testing of various mutations at residue 172 of PvPGIP2_5-8 with PGs using the Y2H system. After 48 hours, the OD of the yeast harboring various mutations mated to (a) BD-AnPG2(b) BD-BcPG1, (c) BD-BcPG2, and (d) BD-FmPG3 was measured. Each column represents the mean values of five biological replicates, and the error bars represent the standard deviation of the replicates. The lower limit shows the activity level of the negative control. G indicates the tPvPGIP1_5-8, and V indicates the tPvPGIP2_5-8. The wild type residues for PvPGIP1 and PvPGIP2 are G and V, respectively, and highlighted by the blue and orange bars, respectively.