Figure 2. Verification of PG-PGIP interactions using the Y2H system. Growth curves of Y2H yeast strains were grown in -HTL dropout media harboring various PGs with (a) full length AD-PvPGIP1 and(b) full length AD-PvPGIP2. The positive control is a yeast strain harboring BD-AnPG2 with AD-PvPGIP2 and grown in -LT dropout media, while the negative control is a yeast strain harboring BD-BcPG2 with AD-HAB1, a protein not known to interact with PGs, grown in -HTL dropout media. The curves represent the mean values of three biological replicates, and the error bars represent the standard deviation of the replicates.