Figure 6. Testing of various mutations at residue 172 of
PvPGIP2_5-8 with PGs using the Y2H system. After 48 hours, the OD of
the yeast harboring various mutations mated to (a) BD-AnPG2(b) BD-BcPG1, (c) BD-BcPG2, and (d) BD-FmPG3
was measured. Each column represents the mean values of five biological
replicates, and the error bars represent the standard deviation of the
replicates. The lower limit shows the activity level of the negative
control. G indicates the tPvPGIP1_5-8, and V indicates the
tPvPGIP2_5-8. The wild type residues for PvPGIP1 and PvPGIP2 are G and
V, respectively, and highlighted by the blue and orange bars,
respectively.