Figure 5. Sequence-function correlation of tPvPGIP2_5-8. tPvPGIP_5-8 containing different combinations of the three residues differing between PvPGIP1 and PvPGIP2 were tested using a Y2H system.(a) shows the three differing residues between PvPGIP1 and PvPGIP2, highlighted in yellow. Each mutant contains a residue from either PvPGIP1 or PvPGIP2 different at LRR5, LRR7, and LRR8, named sequentially as shown in (b) tPvPGIP2_5-8 “221,”(c), tPvPGIP2_5-8 “212,” (d) tPvPGIP2_5-8 “221,”(e) tPvPGIP2_5-8 “122,” (f) tPvPGIP2_5-8 “112,” and (g) tPvPGIP2_5-8 “121.” The positive control yeast was harboring BD-AnPG2 and AD-PvPGIP2 grown in -LT dropout media, while the negative control was yeast harboring BD-BcPG2 and AD-HAB1 grown in -HTL dropout media. The curves represent the mean values of three biological replicates, and the error bars represent the standard deviation of the replicates.