Fig. 3
Effect of sub-zero temperatures on the freezing tolerance of four selected accessions. Freezing tolerance of acclimated and non-acclimated samples was evaluated by measuring electrolytic leakage after exposing detached leaves to sub-zero temperatures. Acclimation occurred at 4°C. The graphs show estimates of LT50 andLT100 values which are derived from a logistic function fitted to the measured data (shown by data points). Empty points present measured data, filled points show mean values of measured data. Dotted lines indicate the LT50 values