2.5 Yeast one-hybrid assay
For yeast one-hybrid (Y1H) assay, a fragment comprising 2000 bp of theOsMYC2 promoter region (from -2000 to -1 relative to the start codon) was amplified and cloned into the pAbAi vector. The ORF ofOsPHR2 was inserted into the pGADT7-Rec2 (AD) vector. These constructs or the corresponding empty vectors were co-transformed into the yeast strain Y1HGold and incubated at 30 °C on SD medium lacking Leu, then spotted on selective media containing 300 ng/ml Aureobasidin A (AbA, Clontech). The primers used are provided in Table S1.