2.9 Primary root growth inhibition assay
The
MeJA-mediated root growth inhibition assay was performed as described
(Yang et al. , 2012; He et al. , 2020). Sterilized seeds
were incubated on one-half MS medium with 0.35% agar and supplemented
with 0 or 20 μM MeJA (TCI), then incubated in a growth chamber at 30 °C
with 8 h light followed by 25 °C with 16 h darkness. Four days later,
root lengths of seedlings were determined. For each treatment, at least
20 seedlings for each plant line were treated and measured. Two
independent experiments were performed.