2.7 Dual-luciferase assay
The dual-luciferase (dual-LUC) assay was performed as described (Heet al ., 2021). The 2 kb promoter region of OsMYC2 was
inserted into the pGreenII 0800-luciferase (LUC) vector as the reporter,
while OsPHR2-Flag and GFF-Flag were cloned into the pCAMBIA1300 vector
as effectors. Effectors and reporter were transformed intoAgrobacterium tumefaciens strain GV3101 and co-infiltrated
into N. benthamiana leaves. At 44-48 h after infiltration, 1 mM
luciferin was sprayed onto the leaves, and the plants were kept in the
dark for 5 min. Afterwards, LUC luminescence was captured using an
imaging system for living plants (Lumazone PyLoN 2048B, USA). The
primers used are listed in Table S1.