COI and ddRAD datasets

211 COI sequences of H. bicuspis were successfully sequenced. The final alignment was 530 base pairs (bp) long, of which 132 bp were parsimony-informative. Stop codons or indels were not observed. 52 of 78 sequenced ddRAD libraries were of sufficient quality for further analyses. The two ddRAD datasets comprised 1723 and 2993 loci with 18,999 and 21,697 bp, and the vast majority of loci yielded one or more SNPs (Table 1).