Sample collection
2 ml venous blood from each rat’s eye socket was collected in the 1st, 3rd, 5th, and 6th courses of treatment and was kept in estrogen receptor tubes at 4°C. When blood coagulation occurred, the tubes were placed in a centrifuge. After centrifugation, the supernatant was collected and kept in a freezer at -20°C. Rat vaginal smears were collected simultaneously for H & E staining, which was done in Guangxi National Hospital and observed by the microscope. All rats were sacrificed by cervical dislocation under anesthesia.