3.7 Technical Validation
To assess the quality of the assembled genome, its accuracy and
completeness were evaluated using BUSCO analysis and the short-read
mapping method (Simão et al., 2015). The integrity and completeness of
the sampled M. japonicus genome was evaluated using BUSCO v4.1.2
based on the arthropoda_odb10 database. We found that 89.4% and 1.0%
of 1,013 BUSCO genes are monitored, while BUSCO type genes were
partially revealed in the genomic database. Burrows-Wheeler Aligner
(BWA) (version 0.7.8-r455) (Li & Durbin, 2009) was used to align the
next generation sequencing short reads to the genome and more than
89.44% of the reads were reliably assigned, representing a relatively
high proportion of the short-view sequencing data.