3.2 Completeness of the assembly
The quality of assembly was evaluated by Illumina short reads mapping, for which the 88.53% coverage showed that the relative completeness of the genome assembly (Table S1). We identified 9,679,458 homozygous SNPs, which indicated a low homozygous rate (0.0099%) and demonstrated that the genome assembly was highly accurate at the single-base level (Table S2).
The BUSCO assessment revealed 87.6% of the 1,066 Arthropoda BUSCOs were identified in the assembled genome (Table S3). Meanwhile, according to the CEGMA analysis, 221 (89.11%) conserved genes were identified in theM. japonicas genome (Table S4). These results indicated that the genome assembly had good coverage and completeness.