Neutralising Antibodies
Microneutralization assay
SARS-CoV-2 isolate CoV/Australia/VIC01/202039 passaged in Vero cells was stored at −80 °C. Serial two-fold dilutions of heat-inactivated plasma were incubated with 100 TCID50 of SARS-CoV-2 for 1 h and residual virus infectivity was assessed in quadruplicate wells of Vero cells; viral cytopathic effect was read on day 5. The neutralizing antibody titer was calculated using the Reed/Muench method40.