2.3 Whole genome sequencing of M. bovis from deer
DNA samples of M. bovis isolates from deer were sent to Oswaldo Cruz Institute (Fiocruz), Manguinhos, Rio de Janeiro, Brazil, for WGS. The concentration and quality of DNA were measured using Qubit fluorometer (Invitrogen, California, USA) and Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Massachusetts, USA). Paired-end genomic libraries were constructed using Nextera DNA Flex Library Prep kit (Illumina, California, USA), according to the manufacturer’s instructions. Genomic libraries were then sequenced in HiSeq 2500 platform (Illumina) using HiSeq Rapid SBS Kit V2 (200 cycles). Illumina sequencing reads were made available in the Sequence Read Archive (SRA), NCBI (Appendix Table 1).