Nematicidal assay on extracts from PA-treated plants
Extracts for nematicidal assays were prepared in the same manner as for
UPLC-MS/MS profiling. Briefly, ground tissue was extracted with 5 ml
methanol g-1 tissue (30 minutes, RT), after which the
extract was filtered, dried in a rotary evaporator (35°C), dissolved in
0.5 ml water g-1 tissue and defatted with cyclohexane.
A two-fold dilution series (from 2:1 to 1:8 mass-to-solvent ratio) was
prepared, and ±100 M. incognita J2s were incubated in 600 µl
diluted extract. Nematode motility was evaluated after 4, 24 and 48 h.
Four biological replicates, each consisting of 5-6 tomato plants, were
used per treatment.