Figure legend
Figure 1. AIV subtypes detected in water samples by country. A
total of 21 subtypes from nine countries were isolated between 2003 –
2020. The H9N2 subtype was the most reported (40.17%, n=94), followed
by the H5N1 (23.07%, n=54) and the H5N6 (8.97%, n=21). The world map
shows the number of AIV subtypes reported by country; red, orange, and
yellow areas indicate high, medium, and low subtype diversity,
respectively. The map was created online athttps://mapchart.net.
Figure 2. The number of AIV sequences detected
in water samples by sampling site. A) live bird markets (LBM), B) wild
bird habitats (WBH), C) poultry farms (PF), D) unknown (UNK) sampling
site. Red, orange, and yellow areas in the map indicate high, medium,
and low number of AIV sequences, respectively. The pie charts show the
proportion of each subtype by country in each sampling site. The bar
chart presents the number of AIV sequences by country and sampling site.
The map was created online athttps://mapchart.net.
Figure 3. Number of AIV sequences by water source and subtype.A) Live bird market, B) Poultry farm. Carcass-wash water (CWW), duck’s
environmental water (DEW), drinking water trough (DW), hand-wash water
(HWW), poultry cage water (PCW), sewage (SW), turkey barn water (TBW),
and unknown (UNK). The highest subtype diversity was found in DW from
live bird markets, and in DEW from a poultry farm.
Figure 4. Circular phylogenetic tree of hemagglutinin
(HA) gene sequences of AIV detected in water samples between 2003 –
2020 worldwide . Viral sequences are color-coded by subtype. Branches
are color-coded by sampling site (red= live bird market (LBM), blue=WBH
(wild bird habitat), purple= poultry farm (PF), and black= unknown
(UNK)). The tree was generated with the Maximum-likelihood method using
MEGA X and edited with FigTree v1.4.4 software.