2.8 Statistical analysis
The statistical analysis of LC-MS profiling data was performed with
MetaboAnalyst 5.0 tool (Chong et al., 2019). Differences in anthocyanin
levels among the light treatments were analyzed by one-way ANOVA
followed by Turkey’s post-hoc test. Statistically significant
differences between the light treatments analyzed by qRT-PCR were
determined by independent samples t- test in SPSS Statistics
program v26 (IBM corporation, New York, NY, USA). Pearson’s correlation
matrices were used to visualize the statistical relationship from
correlation coefficients between selected gene expression levels and
dominant anthocyanin concentrations (delphinidin 3-galactoside and
delphinidin 3-arabinoside) with p-value set to <0.05. ANOVA
and Pearson’s correlation analysis were performed using Origin Pro
software v2020b (Origin Lab Corporation, Northampton, MA, USA).