2.4 Detection of cytokines secreted in PBMCs culture
supernatants
Cytokine expression levels in the supernatants of stimulated cultures
were quantified by multiplex bead-based fluorometric immunoassay
(Milliplex, Millipore) using Luminex xMAP technology (Luminex 200,
Bio-Rad) at the Swiss Institute for Allergy and Asthma Research (SIAF)
in Davos, Switzerland. Values out of range were either marked as the
lowest (those on a lower end) or the highest (those on a higher end)
detectable values. The panel used contained IFNα2, IFNγ, IFNλ-2, IL-1β,
IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-23A, IL-25,
IL-27, IL-33, CCL3, CCL4, CCL5, CXCL8, CXCL10, TNF-α. The T-cell subset
classification of all measured cytokines can be found in Online
Supplementary Table 1.