Genomic DNA library construction and sequencing
The plantlet GM15 generated by in vitro anther culture and regeneration system, was selected for genome sequencing. Genomic DNA was extracted using the Qiagen DNeasy Plant Mini Kit. DNA quality was evaluated by agarose gel electrophoresis and its quantity determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). The short-insert PCR-free genomic DNA library (300-500 bp) was constructed following the manufacturer’s protocol (Illumina Inc., San Diego, CA, USA) for paired-end sequencing on the Illumina HiSeq X Ten sequencer. For SMRT sequencing, the 20-kb genomic DNA library was costructed following the manufacturer’s protocol (Pacific Biosciences, Menlo Park, CA, USA). The detailed description in File S1.