Figure 5: RT-qPCR analysis of selected genes in Bombus impatiens workers’ brain and fat body in the presence of the queen and the brood. Expression levels of selected genes from RNA-seq analysis were examined in workers’ brain and fat body tissues. Pair of newly emerged workers were assigned to four treatments and kept for three days with the queen (CQ), young brood (CB), the queen and young brood (CBQ), or alone (C). Different letters above columns indicate statistical differences at α =0.05. Data are presented as means ± S.E.M.