Figure 7. Indirect ELISA analysis of A. hydrophilaspecific IgM antibody. Fish sera were collected on day 14 and dilutions with 1:256 were used to test for antigen specific IgM. Data were expressed as mean absorbance at OD450nm with a SEM bar (A). One dot represents one biological replicate (n = 9 in group Ah + no phage, n = 10 in other groups). Differences between groups were tested using log-rank test (B). “*” denotes significant difference (p< 0.05), and “ns” means not significant.