Chemicals and reagents
Dimethylsulfoxide (DMSO), chlorzoxazone, (+)-N-3-benzylnirvanol,
ketoconazole, 4-methylpyrazole hydrochloride, quinidine sulphate,
sulfaphenazole, ticlopidine hydrochloride, montelukast,
4-methylaminoantipyrine hydrochloride (4-MAA), 4-aminoantipyrine (4-AA),
4-acetylaminoantipyrine (4-AAA) and 4-formylaminoantipyrine (4-FAA) were
obtained from Sigma-Aldrich (Buchs, Switzerland). Tizanidine
hydrochloride, (S)-efavirenz, flurbiprofen, omeprazole, metoprolol,
paclitaxel, 6’-hydroxychlorzoxazone, 8’-hydroxyefavirenz,
4’-hydroxyflurbiprofen, α-hydroxymetoprolol, 5’-hydroxyomeprazole,
6α-hydroxypaclitaxel, hydroxytizanidine, furafylline, ciprofloxacin
hydrochloride, fluconazole, chlorzoxazone-d3, efavirenz-d5,
flurbiprofen-d3, metoprolol-d6, midazolam-d6, omeprazole-d3,
paclitaxel-d5, and tizanidine-d4, 4-MAA-d3, 4-AA-d3, 4-AAA-d3,
ciprofloxacin hydrochloride-d8 and fluconazole-d4 were purchased from
Toronto Research Chemicals (Toronto, Canada). Midazolam was provided by
Roche (Basel, Switzerland) and α-Hydroxy-midazolam was acquired from
Lipomed (Arlesheim, Switzerland). Corning rhCYP1A2 Supersomes (1
nmol/mL, Lot: 9095001), Corning rhCYP2B6 Supersomes (2 nmol/mL; Lot:
9268001), Corning rhCYP2C8 Supersomes (2 nmol/mL, Lot: 7278001), Corning
rhCYP2C9*1 (Arg144) Supersomes (2 nmol/mL, Lot: 9277002), Corning
rhCYP2C19 Supersomes (2 nmol/mL, Lot: 9275001), Corning rhCYP2D6*1
Supersomes (1 nmol/mL, Lot: 9274002), Corning rhCYP2E1 Supersomes (2
nmol/mL, lot: 9290001), Corning rhCYP3A4 Supersomes (2 nmol/mL, Lot:
1006003), NADPH regeneration solution A (26 mM NADP+, 66 mM
glucose-6-phosphate, and 66 mM MgCl2 in H2O, Lot:
9288003) and B (40 U/mL glucose-6-phosphate dehydrogenase in 5 mM sodium
citrate, Lot: 8024003) were obtained from Corning Life Sciences B.V.
(Amsterdam, The Netherlands). HPLC grade methanol, HPLC grade water, and
formic acid were purchased from Merck (Darmstadt, Germany).