Chemicals and reagents
Dimethylsulfoxide (DMSO), chlorzoxazone, (+)-N-3-benzylnirvanol, ketoconazole, 4-methylpyrazole hydrochloride, quinidine sulphate, sulfaphenazole, ticlopidine hydrochloride, montelukast, 4-methylaminoantipyrine hydrochloride (4-MAA), 4-aminoantipyrine (4-AA), 4-acetylaminoantipyrine (4-AAA) and 4-formylaminoantipyrine (4-FAA) were obtained from Sigma-Aldrich (Buchs, Switzerland). Tizanidine hydrochloride, (S)-efavirenz, flurbiprofen, omeprazole, metoprolol, paclitaxel, 6’-hydroxychlorzoxazone, 8’-hydroxyefavirenz, 4’-hydroxyflurbiprofen, α-hydroxymetoprolol, 5’-hydroxyomeprazole, 6α-hydroxypaclitaxel, hydroxytizanidine, furafylline, ciprofloxacin hydrochloride, fluconazole, chlorzoxazone-d3, efavirenz-d5, flurbiprofen-d3, metoprolol-d6, midazolam-d6, omeprazole-d3, paclitaxel-d5, and tizanidine-d4, 4-MAA-d3, 4-AA-d3, 4-AAA-d3, ciprofloxacin hydrochloride-d8 and fluconazole-d4 were purchased from Toronto Research Chemicals (Toronto, Canada). Midazolam was provided by Roche (Basel, Switzerland) and α-Hydroxy-midazolam was acquired from Lipomed (Arlesheim, Switzerland). Corning rhCYP1A2 Supersomes (1 nmol/mL, Lot: 9095001), Corning rhCYP2B6 Supersomes (2 nmol/mL; Lot: 9268001), Corning rhCYP2C8 Supersomes (2 nmol/mL, Lot: 7278001), Corning rhCYP2C9*1 (Arg144) Supersomes (2 nmol/mL, Lot: 9277002), Corning rhCYP2C19 Supersomes (2 nmol/mL, Lot: 9275001), Corning rhCYP2D6*1 Supersomes (1 nmol/mL, Lot: 9274002), Corning rhCYP2E1 Supersomes (2 nmol/mL, lot: 9290001), Corning rhCYP3A4 Supersomes (2 nmol/mL, Lot: 1006003), NADPH regeneration solution A (26 mM NADP+, 66 mM glucose-6-phosphate, and 66 mM MgCl2 in H2O, Lot: 9288003) and B (40 U/mL glucose-6-phosphate dehydrogenase in 5 mM sodium citrate, Lot: 8024003) were obtained from Corning Life Sciences B.V. (Amsterdam, The Netherlands). HPLC grade methanol, HPLC grade water, and formic acid were purchased from Merck (Darmstadt, Germany).