Monocyte isolation
Monocytes were isolated from PBMC by incubation with anti-CD14 antibody coupled to magnetic microspheres (EasySep Human CD14 Positive Selection Kit II), the tube with the mixture was placed on an EasySep magnet for immunomagnetic positive selection of CD14+ cells (StemCell). Monocytes were used for cell culture and an aliquot stored in RNAlater (Sigma Aldrich) for RNA isolation.