Metabolic stimuli promote differentiation to metabolic,
classical, and alternative macrophages.
We aimed to investigate the effects of high concentrations of molecules
such as, glucose, insulin, and palmitate, in the polarization of
macrophages into different phenotypes. Once the monocytes were purified
from healthy and dyslipidemia subjects, cells were incubated with the
metabolic stimuli mentioned above, dyed, and analysed by flow cytometry
(Fig 4A). We obtained different macrophages subsets such as M1
(CD14+CD68+CD80+),
M2
(CD14+CD163+CD206+)
and MMe
(CD14+ABCA1+CD36+PLIN2+),
being M1 the most abundant subset among the polarized cells (p= 0.0004;
Fig 4B).