Metabolic stimuli promote differentiation to metabolic, classical, and alternative macrophages.
We aimed to investigate the effects of high concentrations of molecules such as, glucose, insulin, and palmitate, in the polarization of macrophages into different phenotypes. Once the monocytes were purified from healthy and dyslipidemia subjects, cells were incubated with the metabolic stimuli mentioned above, dyed, and analysed by flow cytometry (Fig 4A). We obtained different macrophages subsets such as M1 (CD14+CD68+CD80+), M2 (CD14+CD163+CD206+) and MMe (CD14+ABCA1+CD36+PLIN2+), being M1 the most abundant subset among the polarized cells (p= 0.0004; Fig 4B).