Soluble cytokine quantification
To measure multiple soluble cytokine levels in serum and culture
supernatants from dyslipidemia patients and control subjects, we
performed a cytometric bead assay (BD CBA, inflammatory cytokine array).
Briefly, serum and supernatants samples were incubated with capture
beads and detector antibodies; then, sandwich complexes were formed with
these reagents and the analyte. These complexes were measured by flow
cytometry, according to size and fluorescence characteristics of both
the beads and the detector antibody, in an Accuri C6 cytometer using
FCAP array software for analyses.