Monocyte isolation
Monocytes were isolated from PBMC by incubation with anti-CD14 antibody
coupled to magnetic microspheres (EasySep Human CD14 Positive Selection
Kit II), the tube with the mixture was placed on an EasySep magnet for
immunomagnetic positive selection of CD14+ cells
(StemCell). Monocytes were used for cell culture and an aliquot stored
in RNAlater (Sigma Aldrich) for RNA isolation.