Statistical analysis
Data were analysed with GraphPad Prism, 5.01 software. Data were
analysed for normality with the Pearson test, Kolmogorov-Smirnov test,
D’Agostino, and Shapiro-Wilk test. Flow cytometry data were evaluated by
paired or unpaired Student t-test in case of a normal distribution;
otherwise, we used the Mann-Whitney U test or Wilcoxon signed-rank
sum-test. In some cases, data were analysed by one-way ANOVA, Friedman
test, or two-way ANOVA test. The analysis of correlations between
variables was based on Spearman’s rank test. P <0.05 was
considered statistically significant. The data are shown as mean and SD,
or median with interquartile range (IQR).