2.5 Mosquitoes
The Poza Rica Ae. aegypti strain was colonized in 2012 from collections in the state of Veracruz, Mexico(Vera-Maloof, Saavedra-Rodriguez, Elizondo-Quiroga, Lozano-Fuentes, & Black IV, 2015). The Cx. tarsalis Kern National Wildlife Refuge (KNWR) colony(Oviedo, Romoser, James, Mahmood, & Reisen, 2011), established in 1952, was obtained from the Centers for Disease Control and Prevention (Fort Collins, CO). Mosquito colonies were maintained at 24-26oC (Culex ) or 28oC (Aedes ) at 12:12 light:dark cycle; adults were fed water and sucrose ad libitum . Larvae were reared on ground TetraMin fish food (http://www.tetra-fish.com/).