3.6 Culture enrichment of transconjugant sewage community
resulted in single genera domination
Lastly, we assessed the taxonomy of the sorted and enriched
transconjugant sewage community, in order to understand which hosts
enable stable replication and maintenance of the two plasmids, and hence
are able to support all three stages of plasmid host range. For all
samples, enrichment promoted single-genus dominance, yet marked
differences were observed between the two plasmid-associated sewage
community transconjugant pools. While Serratia was primarily
enriched in the transconjugant sewage community/R27 ( = 99.2%), Aeromonas dominated the transconjugant sewage community/pB10 pool
(= 99%) (figure 5b).