Figure 4. Phylogeny and relative abundance of the transconjugant sewage community. a) Phylogenetic tree, based on Maximum Likelihood, for the 65 ASVs present (n taxa) in the transconjugant sewage community after data cleaning. The ASVs are named according to genus annotation. As the legends at the left indicate, ASVs are colored according to family. ASVs were only assigned to Gram-negative phylogroups indicated by Gram (+/-). The log10 relative abundance of each ASV in the recipient sewage community (SC) is shown as the white to blue scaled heatmap. A pseudo count of 1 read was added to the OTU table before log transformation. The bar chart shows the distribution of mean % relative abundance for each transconjugant sewage community (pB10 and R27) between the ASVs, colored according to the plasmid. Taxonomy of clades is indicated by the text on branches, the phyla Proteobacteria, Bacteroides, the classes Flavobacteriia, Gammaproteobacteria, Betaproteobacteria and orders Enterobacteriales Pseudomonadales, Burkholderiales and Flavobacteriales. The bar plot b) shows the mean % relative abundance (% rel. abundance on the y-axis) between families in the two transconjugant sewage communities (Plasmid), plotted on the x-axis. Colored according to families, this legend is shared between figure a) and b). Error bars represent standard deviation.