Author contributions:
All work was supervised by S.M. and S.J.S.; A.K.O., R.P.R., A.D., B.F.S., J.S.M. and S.J.S. planned the experiments; A.K.O. carried out plasmid and strain tagging under the guidance of J.S.M.; Vector construction for attTn7 chromosomal tagging where carried out by M.F.H.; A.K.O carried out sampling, planned by A.D and B.F.S.; A.K.O. carried out conjugation assays, flow cytometry and cell sorting under the guidance of R.P.R., J.S.M. and S.J.S.; DNA extraction and sequencing were carried out by A.K.O and J.N.; Sequence analysis and other data treatment were carried out by A.K.O under the guidance of J.N. and J.R.; A.K.O. wrote the article and all authors contributed to the manuscript with feedback and input.