It is additonally possible to attatch a UV LED for flouresecne imaging.
Note that although the DOME is capable of bright-field and fluorescence illumination, these features were not used in the experiment presented here. A camera (Camera Module V2, Raspberry Pi) sits on the base of the imaging column pointed upwards at the sample stage. Optics such as wavelength or neutral density filters can be added into the optics holder within the imaging column on an application specific basis. For the lower magnification configuration, the imaging column ends with a 9× tube lens (Eyepiece Cell Assembly, Edmund Optics) screwed into a threaded cylindrical casing. For higher magnification applications the cylindrical section is extended, ending in an RMS thread to fit a standard microscope objective (finite conjugated 10X Semi-Plan Standard Objective, Edmund Optics). While the length of this lens piece is specific to the lenses used here, due to the modular nature of the DOME it would be trivial to adjust this dimension to suit alternative optics. Positioning both the camera and projector perpendicular to the sample stage results in significant lens flare through which imaging is difficult. To circumnavigate this the projector is angled at 10°, positioning the bright spot created by the light source of the projector out of the camera field of view (FOV). The exact spacing between critical optical components is given in Figure 6.