In vitro systems serve as compact and manipulate models to investigate
interactions between different cell types. A homogeneous population of
cells predictably and uniformly responds to external factors. In a
heterogeneous cell population, the effect of external growth factors is
perceived in the context of intercellular interactions. Indirect cell
co-cultivation allows one to observe the paracrine effects of cells and
separately analyze cell populations. The article describes an
application of custom-made cell co-cultivation systems based on protein
membranes separated from the bottom of the vessel by the 3d printed
holder or kept afloat by a magnetic field. Using the proposed
co-cultivation system, we analyzed the interaction of A549 cells and
fibroblasts, in the presence and absence of growth factors. During
co-cultivation of cells, the expression of genes of the activation for
epithelial and mesenchymal transitions decreases. The article proposes
the application of a newly available system for the co-cultivation of
different cell types.