2.1. Materials
Laminin, anti-β-tubulin III antibody produced in rabbits were purchased from Sigma-Aldrich (Steinheim, Germany). Lyso-tracker green, tubulin-tracker deep red staining kit for living cells, poly-D-lysine (PDL), penicillin-streptomycin solution (100X) and Hoechst 33342 staining solution were provided from Beyotime (Shanghai, China). Neurobasal medium, sodium pyruvate (100 mM), B-27TMsupplement (50X) serum free, L-Glutamine 200 mM (100X) were purchased from Gibco (Thermo Fisher Scientific, Ireland). The goat polyclonal secondary antibody to rabbit IgG-H&L (Alexa Fluor®488) was from Abcam (Cambridge biomedical campus, UK).