Figure 3. The electrophysiological properties of primary neurons transfected or untransfected with plasmids. (A) Untransfected neuron and (B) transfected neuron under the whole-cell patch-clamp recording. (C) A current injection procedure. (D) Fluorescence imaging of transfected neurons. Dotted lines indicate the position of the micropipette. (E) The evoked action potentials correspond to untransfected neurons (A). (F) The evoked action potentials correspond to transfected neurons (B). Scale bar: 20 μm.