2.6.1. Axon width measurement
The axon width was measured by Fiji (open source https://fiji.sc/) and Origin software (Origin Lab Corporation, Northampton, Ma, USA). Firstly, the ordinal operation ImageJ-Analytic-Set Scale was used to adjust the scale bar, and then ImageJ-Analyze-Plot Profile click could extract the fluorescence intensity value (gray value) of neuronal axons. These data were stored in a text file and then imported into the Origin to draw a line chart. Ordinal click Analysis-Fitting-Nonlinear Curve Fit-Open Dialog-Gauss was used to perform the Gaussian fitting. Finally, the fitted curve was selected, followed by orderly clicking Analysis-Mathematics-Integrate-Open Dialog, checked ”connect endpoint as a baseline”, and then the “dx” in the Results Log window was the width of axons in the raw image.