Microscopy
Light Microscopy : Seeds were examined under a dissecting
microscope (Nikon SMZ 1500) attached to a digital camera (Nikon Digital
sight DS-5M) at 2× zoom range.
Transmission Electron Microscopy (TEM) : See sections (150 nm
thick) were prepared using a microtome (Reichert-Jung ultra, Leica
Microsystems Inc.), fixed sequentially in glutaraldehyde (3% v/v in 0.1
M sodium cacodylate buffer at pH 7.2) and 1% OSO4 (in
sodium cacodylate buffer), stained with uranyl acetate, embedded in
Epon-Araldite resin (Polyscience Inc.) and then mounted on a 200-mesh
copper grid. TEM images (5000-50,000 × magnification) were obtained
using a Hitachi HT7700 electron microscope (Hitachi-High Technologies
Inc.) set at high contrast field and 80 kV. Oil droplet diameter and the
number of droplets per unit area were recorded using ImageJ Fiji
software (Life-Line version 2014; https://imagej.net/software/fiji/).