Real-time quantitative reverse
transcription-PCR
TRIzol reagent (Invitrogen, USA) was used to extract total RNA from
peanut root. Then, RNase-free DNase I (Takara, Japan) was used to remove
genomic DNA contamination. cDNA was synthesized using ReverTra Ace
Reverse Transcriptase (Toyobo, Japan). SYBR Premix Ex Taq (Perfect Real
Time) reagent (Takara, Japan) and a real-time PCR detection system
(CFX96; Bio-Rad, USA) were used to perform real-time quantitative PCR.
The primers used for real-time quantitative PCR are listed in Table S1.