Up-regulation of AhYSL1 expression in root by PDMA application
The expression of Fe transporters under the different treatments is displayed in Fig. 4. AhYSL1 expression in roots treated with PDMA and PDMA–Fe was 1.8-fold and 2.5-fold the expression in the control, respectively, but no difference was detected with EDTA–Fe treatment (Fig. 4a). However, no significant differences in AhFIT andAhNRAMP1 expression were detected (Fig. 4b, c). These results show that PDMA application promoted Fe absorption by roots, as represented by up-regulated AhYSL1 transporter expression (rather than AhFIT or AhNRAMP1 transporter expression).