Inhibition of ferric reductase activity and AhIRT1expression by PDMA application
The physiological and underlying
molecular responses to Fe deficiency in roots were inhibited following
PDMA application. As shown in Fig. 3a, the three treatments greatly
reduced ferric reduction activity compared with the control, and the
plants treated with PDMA–Fe exhibited a lower level than plants treated
with PDMA and a higher level than plants treated with EDTA–Fe.
Additionally, AhIRT1 expression in roots was decreased by 58%,
55%, and 56% following treatment with PDMA, PDMA–Fe, and EDTA–Fe
compared with the control, respectively (Fig. 3b). These results
indicate that the available Fe level in the rhizosphere was much higher
following PDMA, PDMA–Fe, and EDTA–Fe treatment than in the control
plants.