Real-time quantitative reverse transcription-PCR
TRIzol reagent (Invitrogen, USA) was used to extract total RNA from peanut root. Then, RNase-free DNase I (Takara, Japan) was used to remove genomic DNA contamination. cDNA was synthesized using ReverTra Ace Reverse Transcriptase (Toyobo, Japan). SYBR Premix Ex Taq (Perfect Real Time) reagent (Takara, Japan) and a real-time PCR detection system (CFX96; Bio-Rad, USA) were used to perform real-time quantitative PCR. The primers used for real-time quantitative PCR are listed in Table S1.