Assessing bacteria that distinguish bee species and are core to
Xylocopa
To identify bacterial taxa that differ between host species and tissue
type, we compared relative abundance of ASVs between bee species and
tissues using DESeq2 (Love et al. 2013), with a false discovery rate FDR
<0.05.
To identify if a core bacterial community was shared between the guts ofX. sonorina and X. tabaninformis bees, individual ASVs
were first collapsed at the genus_species level usingtax_glom(). The function eulerr::core_members() was then
used to identify genus_species bins present at ≥ 0.01% relative
abundance in at least 95% of gut samples and heatmaps constructed to
visualize the number of shared core taxa.