Comparative analysis
A total of 13 protein coding genes (PCGs) could be consistently
annotated across all genomes generated in this study. The PCGscox1 , cox2 , cox3 , atp6 , atp8 ,atp9 , nad1 , nad2 , nad3 , nad4 ,nad4L , nad5 and nad6 from 13 fungal strains were
concatenated and aligned using MAFFT v7.221 (Katoh & Standley, 2013)
and a small number of regions containing gaps were eliminated. PhyML
v3.3.20180214 (Guindon et al., 2010) was used to construct the
maximum-likelihood tree using the LG method with 250 bootstrap
replicates. The tree was visualised in Geneious Prime. Whole
mitochondrial genome alignments were performed using the Mauve v1.1.3
(Darling, Mau, & Perna, 2010) plugin in Geneious Prime.