Complete genome sequencing
The total RNA was used to prepare libraries for Next Generation
Sequencing (NGS). Six genomes were obtained following the sequence
independent single primer amplification (SISPA) method and sequenced by
Ion Torrent S5 (Thermo Fisher Scientific, Rodano, Italy) as previously
described (De Sabato et al., 2018b) at Istituto Superiore di Sanità
(FAST service). Two complete genomes were obtained at Istituto
Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”
as previously described (Pierini et al., 2021) by Istituto
Zooprofilattico Sperimentale dell’Abruzzo e del Molise ”G.
Caporale”(Teramo, Italy). The reads obtained by the NGS run were quality
checked and analysed by Galaxy Aries (https://aries.iss.it) (Knijn et
al., 2020). The complete genomes were submitted to the NCBI database
(https://www.ncbi.nlm.nih.gov): 132 (Acc. N° OK340737), WB03VT2016 (Acc.
N° OK340738), WB01VT2016 (Acc. N° OK340739), WB110VT2017 (Acc. N°
OK340740), WB171VT2017 (Acc. N° OK340741), WB119VT2017 (Acc. N°
OK340742), 55863/2018 (Acc. N° OK429318), 47272/2017 (Acc. N° OK429319).