Vaccine Trial of the Escherichia Coli-Expressed Vaccine and Inactivated
Whole Particle Vaccine against Infectious Pancreatic Necrosis Virus in
Rainbow Trout
Abstract
ABSTRACT Infectious pancreatic necrosis (IPN) is a highly contagious
disease causing high mortality in juvenile trouts. Since there is no
effective way to treatment against IPNV, early diagnosis and prevention
play an important role in combating the disease. The different types of
IPNV vaccines (inactive, live, recombinant, DNA, etc) have been produced
from local isolates and have been used in developed countries. In
Turkey, there is no commercial licensed vaccines against IPNV. Due to
this reason, IPNV vaccine is needed in Turkey. The production of
recombinant VP2 subunit vaccine (IPNV-VP2) and inactivated whole
particle virus vaccine (IPNV-WPV) were attempted from selected isolate
belong to sp serotype. For this purpose; the virus was produced in RTG-2
cell line and RT-PCR amplification was performed by using primers with
restriction enzymes. The whole VP2 gene was cloned into a plasmid vector
and VP2 was expressed by using E. coli expression system. A trial was
conducted to determine the immunity ability of IPNV-VP2 and IPNV-WPV in
rainbow trout. Relative percent of Survive (RPS) was detected as 79% in
fish vaccinated with IPNV-WPV and 70% in fish vaccinated with IPNV-VP2.
Thus, we can say that the recombinant vaccine of IPNV-VP2 is almost
protected against IPNV infection as well as the inactive vaccine.