Discussion
S. intermedius abscesses have two presentations one of which being monomicrobial associated with hematogenous spread or are deep-seated and extensive compared with the other two species within its group which were polymicrobial and often more superficial [4]. However, it has been seen that when S. intermedius is found in conjunction with anaerobes in an abscess, there is synergy between the organisms causing enhanced growth [8]. SAG abscesses overall have a longer duration of symptoms than other organisms and the majority of patients need surgical or percutaneous drainage for cure [6]. Interestingly, there appear to be no differences in mortality, duration of antibiotics, or complications compared to non-SAG organisms [6]. Infection with S. intermedius is very rare in previously healthy individuals without any identifiable risk factors [9]. The two most common risk factors are dental manipulation and sinusitis. Others include a history of diabetes, heavy alcohol consumption, congenital heart disease, heart-related conditions, malignancy, periodontal disease, preceding pneumonia, neurological diseases, and COPD [1,5,9].
Infection and abscess formation is started with tissue damage and is then followed by colonization [1]. S. intermedius has surface proteins called Antigens I/II that bind to human lactoferrin, fibronectin, and laminin components that subsequently induce monocytes to release IL8, activating neutrophils leading to a pro-inflammatory state that induces tissue damage and abscess formation [1,8,10]. Hydrolytic enzymes are then released to induce tissue liquefaction and form pus [10]. Hyaluronidase, one of the hydrolytic enzymes released by S. intermedius, has numerous effects. It decreases the viscosity of ground substance and increases the permeability of connective tissue allowing bacterial and toxins to spread while, along with α-N-acetylmuramidase (sialidase), creating a source of nutrients for the bacteria from the degradation products [1, 8]. It has been shown that the higher the concentration of hyaluronidase, the deeper the lesion [8]. It is also involved in biofilm formation, which decreases the organism’s antibiotic susceptibility and protects it from host immunological defenses [1]. It has signal molecules called autoinducers that give it the ability to monitor the concentration of bacterial signals and biofilm formation, allowing it to regulate its pathogenic capabilities like proteolytic and hemolytic enzyme activities, iron acquisition, antibiotic production, carbohydrate metabolism, and biofilm formation [10]. S. intermedius thrives in acidic environments causing the release of iron from host transferrin to facilitate bacterial growth and infectious spread [8]. It also can move to distant sites and rapidly decrease the pH, facilitating the release of iron at this new location for its growth [10]. Some S. intermedius have a polysaccharide capsule, which inhibits phagocytosis and increases its tendency to form abscesses [1, 10]. Encapsulated strains have larger abscesses, earlier spontaneous drainage, and a higher lethality [8]. Superantigens also play an important role in the S. intermedius’s virulence by stimulating the release of proinflammatory mediators by activating glial cells and peripheral immune cells, causing a positive feedback loop to recruit and activate new inflammatory cells and glia to create a vicious cycle allowing extensive damage to normal brain tissue [10]. S. intermedius is unique to the other bacteria in SAG due to the expression of intermedilysin (ILY), allowing it to cause cell necrosis with membrane bleb formation [1, 10]. It is an important virulence factor in causing deep-seated abscesses and its expression correlates with strain pathogenicity or with the severity of infections [1, 8]. Isolates found in deep-seated brain abscesses had a 6.2- to 10.2-fold higher expression of ILY than those found in normal habitats like dental plaques; and ily knockout strains showed greatly decreased adherence, invasion, and cytotoxicity of human liver cells [10]. Because of its specificity to S. intermedius, the ILY gene is being used as a specific marker detector via PCR [6,8].
Brain abscesses typically present with intermittent fever, constant headaches, and/or mental status changes with elevated CRP and WBC count [1, 6]. It can occur due to spread from the contiguous focus of infection (sinus, teeth, or middle ear), hematogenous spread from distant focus, or as a result of head trauma or neurosurgery [6,8]. An important primary source of S. intermedius is from a liver abscess, but most patients have multiple risk factors for developing an invasive intracerebral abscess including congenital heart disease, sinusitis, otitis media, and dental caries [8,11]. The majority of brain abscesses are solitary and occur in the frontal lobe [6]. Due to newer antibiotics and earlier detection with CT scans and MRIs, morbidity and mortality have decreased [6]. Pleuro-pulmonary infections are uncommon and usually present with fever, cough, chest pain, and dyspnea with elevated CRP and WBC count [12]. These infections can present as pulmonary abscesses, pneumonia, pleural effusions, pyopneumothorax, and pleural fistula [13]. It occurs due to aspiration of oral secretions, which might be why it is more frequent in the elderly [12]. Aspirate of the pleural fluid would show exudate, with elevated protein, LDH, and WBC count [12]. The prognosis of pleuro-pulmonary infections is generally favorable [12]. Liver abscesses are generally associated with underlying hepatobiliary or pancreatic disease, diabetes, liver transplantation, and colorectal neoplasm [2]. The probable origin is a dental plaque [2]. Symptoms depend on the size of abscesses and the general health of the patient, but often include upper abdominal quadrant pain, high fevers, nausea, and vomiting [13]; and most patients have an elevated WBC count and liver function tests [2]. Less common symptoms include loss of appetite, jaundice, ascites, pleural effusion, and respiratory symptoms [13]. If liver abscesses are untreated, major complications include sepsis and multiorgan dysfunction/failure, rupture of abscess into peritoneal cavity, thrombosis of the portal or hepatic veins, IVC occlusion of the pseudoaneurysm of the hepatic artery, hemophilia, and fistula to the portal vein or suprahepatic veins [2,13]; with a mortality rate of 100% [13]. When treated, these numbers go as low as 2.5% - 14% [14]. Other possible infection sites include the mouth, female genital tract, appendix, and blood stream [11].
Patients with suspected infection by S. intermedius should have the abscess drained and cultured along with blood cultures if there is suspicion of hematogenous spread [4,6]. Abscess culture can be negative due to antibiotic therapy. If there is a negative culture and suspicion is high, gene amplification and sequencing tests may be used to aid in diagnosis and allow for more targeted antibiotic therapy [4]. S. intermedius can also be difficult to detect with conventional microbiology, so a broad range PCR method targeting the 16S ribosomal RNA genes is typically used [14]. Since the ILY gene is specific to S. intermedius, this is the gene sequenced with PCR to detect these S. intermedius infections [6,8]. Brain abscesses can be evaluated with an MRI which would show ring-enhancing lesions [8]. A lumbar puncture should not be done as CSF analysis does not contribute to diagnosis and there is a risk of brain herniation with the increased ICP caused by abscesses [4]. Pleuro-pulmonary infections should get cultures of the pleural effusion via percutaneous lung needle aspiration or transthoracic needle aspiration [12,15]. If pleural effusion is negative, a lung sample can be taken for microscopy and culture [16].
Management and treatment of these abscesses include drainage, surgery, and antibiotics [1]. Antibiotic therapy is based on susceptibility. All SAG are generally susceptible to the usual dose of penicillin, amoxicillin, cefotaxime, or ceftriaxone and have variable susceptibility to tetracycline, clindamycin, and erythromycin [14]. Of the beta-lactams, ceftriaxone is preferred due to its excellent activity and tissue penetration [12]. If the patient has a penicillin allergy, vancomycin is typically used [12]. However, recent penicillin strains have been reported and in cases of high risk of antibiotic resistance, carbapenems, especially ertapenem, are very effective [14, 15]. Most strains are resistant to aminoglycosides and resistance to macrolides and clindamycin are increasing [9,12]. Empiric therapy should depend based on patients’ current condition and whether they have been exposed to antimicrobials within the past three months [9] Fluoroquinolones have also been shown to be efficacious against SAG, but resistance develops quickly so these are not appropriate as empiric therapy [12]. The most commonly used antibiotic regimens currently used are a combination of ceftriaxone and metronidazole or a combination of ceftriaxone, metronidazole, and vancomycin [1]. However, with the use of multiple antibiotic regimens in patients, there is a concern for the emergence of drug-resistant strains, especially with vancomycin and carbapenems [1]. There also have been cases of clinical failure with antibiotics that showed susceptibility in vitro studies. Potential causes of clinical failure can include the inappropriate selection of empiric therapy, inability to penetrate tissue spaces, and inadequate dosing to reach therapeutic concentrations in infected areas [9].
Brain abscesses are managed with a combination of neurosurgical intervention, antibiotics, and eradication of any primary foci [10]. Patients with significant risk factors for surgery or small cerebral lesions <5mm on CT should undergo conservative management with antibiotics [4,10]. In those who can tolerate surgery, CT-guided stereotactic aspiration of abscess can be performed as it is minimally invasive, rapid, and effective in surgical drainage especially if an abscess is small and deep-seated [4]. CT-guided stereotactic aspiration is associated with lower morbidity and mortality and has a better overall prognosis [4]. A more superficial abscess can be treated with craniotomy and excision of abscess [4]. The working group of the British Society of Antimicrobial Chemotherapy recommended that brain abscesses should be treated with a combination of beta-lactam and metronidazole for three to four weeks if surgically resected or four to six weeks if aspirated [10]. Pleuropulmonary infections are managed via drainage of pleural fluid, pleurectomy, thoracotomy, decortication, and debridement [12,13]. Reasons for clinical failure seen in pneumonia complicated by empyema by S. intermedius species include the inability to penetrate pleural space and the inability to reach therapeutic levels in desired areas [17]. Early recognition and timely intervention imp for successful treatment and better prognosis [14]. Liver abscesses are treated with IV broad-spectrum antibiotics, most commonly fluoroquinolones or third-generation cephalosporin in combination with metronidazole, and percutaneous drainage [13]. For abscesses less than 5cm, antibiotics alone can be sufficient; for abscess between 5-7.3 cm with sustains fevers for more than 23-48 hours or suggestion of perforation, a combination of percutaneous drainage and systemic antibiotics should be used; and for abscesses that are larger than 7.3 cm should be considered for surgical drainable due to the risk of being multiloculated and spontaneous rupture [13].