Response Surfaces and Bliss Independence Model for Synergy Analysis
Four 2-compound combinations (Adenine/EDTA-Fe, 6-BAP, EDTA-Fe, Adenine/6-BAP, and HA/SWE) were further analyzed via a 4x4 checkerboard design. The design had 16 different combinations with expanded concentrations (0 – 2x Level 2; except 6-BAP) ranging from 0 to 1 g L-1 for Adenine, 0 to 2 g L-1 for EDTA-Fe, 0 to 1 mg mL-1 for 6-BAP, 0 to 2 g L-1 for Humic Acid, and 0 to 100% for SWE. Subsequently, the measured %Yield and morphological data for each of the 16 combinations were used to generate a response surface and respective 2-dimenstional heatmap for each 2- compoundcombination in MATLAB 2020b (Mathworks, Inc.). The interaction maps of these 2-compound combinations were generated using GraphPad PRISM 9 (GraphPad Software). The %Yield data were uploaded to SynergyFinder using Bliss Independence Model to quantify synergy in the tested region \cite{Ianevski_2020}. The resulting Bliss synergy score data were downloaded and used to generate Bliss Synergy Map using GraphPad PRISM 9 (GraphPad Software).