Mono-Compounds were Broadly Ineffective in Increasing %Yield
The WisDM Green optimization process assessed 3 concentration levels (0 indicates absence of a compound, and 1 and 2 represent two concentration levels) for each compound (Table 5). Concentrations were referenced to previous studies and were carefully selected in an effort to avoid overrepresenting a single compound. The mono-compound experiment was performed by growing red spinach using level 1 and level 2 concentrations of each compound to assess their individual effect in %Yield (N = 3) (Equation 1 and Table 5). All 8 compounds in their level 1 and 2 concentrations had a minimal effect in increasing %Yield, and most compounds resulted in negative %Yield (Figure 5a and 5b). Importantly, these 8 compounds demonstrated concentration-dependent correlations between %Yield and their concentrations. Starch, HA, EDTA-Fe, SWE revealed a positive relationship such that the increase in concentrations resulted in improved %Yield. However, the remaining compounds led to a negative relationship between concentrations and %Yield. These correlations suggested that high concentrations of compounds may not always associate with improved %Yield. Though the compounds were broadly ineffective in increasing %Yield, we aimed to test if pairing them correctly may positively impact %Yield.