Nutritional Analysis
The WisDM Green-designed combinations highlighted in Figure 9a mediated an increase in %Yield of red spinach. The nutrients and elements in red spinach grown with these combinations (Level 2 concentrations) were comprehensively assessed and compared to the control red spinach. Three basic morphological features including shoot length, root length, and leaf size were compared to provide an insight into the baseline characteristics of the plants. Total protein, vitamin C, and critical elemental contents were measured. The full comparisons are illustrated Figure 15. Notably, the optimal WisDM Green-designed combinations demonstrated a substantial increase in absolute Zn content, which is critical to immune system and metabolism function \cite{Wessels_2021}. 6-BAP/HA/SWE/Starch (2nd ranked combination) led to a 15.32±8.42 increase in %Yield, while maintaining an average absolute Vitamin C content at 1.78±0.78 mg, which is 44% higher than the absolute content in control spinach. However, Kruskal-Wallis test followed by Dunn’s post hoc test failed to detect any statistically significant difference in morphological features and absolute nutritional contents between control red spinach and those grown with optimal combinations across all assessments. The results suggested that WisDM Green was able to identify compound combinations that substantially increased the %Yield without compromising the absolute nutritional contents of red spinach. It is important to approach these findings as a proof of concept of the platform as conclusions may only be made with additional nutritional analysis.