Skin tests
Skin prick test (SPT) was performed using undiluted commercially
available ICM solutions, and intradermal test (IDT) was conducted
with10-fold dilutions in 0.9% sterile saline solution. When a SPT was
positive after 20 minutes, and IDT was performed with immediate
readings. Readings at 24 hours of IDT were also conducted to test for
delayed reactions. Saline 0.9% and histamine hydrochloride 10 mg/ml
(ALK-Abello©, Spain) were used as negative and positive controls,
respectively.