Skin tests
Skin prick test (SPT) was performed using undiluted commercially available ICM solutions, and intradermal test (IDT) was conducted with10-fold dilutions in 0.9% sterile saline solution. When a SPT was positive after 20 minutes, and IDT was performed with immediate readings. Readings at 24 hours of IDT were also conducted to test for delayed reactions. Saline 0.9% and histamine hydrochloride 10 mg/ml (ALK-Abello©, Spain) were used as negative and positive controls, respectively.