1. Trophoblast and fibrotic regions: disorganised
trophoblast in pre-eclamptic placenta
H&E stained placental chorion illustrated nuclear accumulations and the
villous tree components (Figure 1A, 1B). As frequently reported in
literature13, 35, EO-PE placenta was characterised by
increased syncytial knotting (Figure 1B) in comparison to term control
tissue (Figure 1A). As depicted in Figure 1, MPM imaging of unstained
tissue (Figures 1C,1D, Figure S1) and stained tissue (Figures 1E-1H)
provided significantly more detailed information about the structural
organisation of the trophoblast. Term control placenta was characterized
by a structured trophoblastic layer (Figure 1C). Nuclei were closely
arranged to each other, and cytoplasmic space was small (inset Figure
1C). Trophoblast of the EO-PE case was disorganised, and numerous
nuclear accumulations were present (Figure 1D and Figure S1B & S2A).
Nuclei were unevenly distributed with larger appearing cytoplasmic
spaces between nuclei (inset Figure 1D). Additionally, excessive
collagen accumulation, i.e., fibrosis, was especially observed in stem
villi of EO-PE placenta (Figures S2A, S2B).