Transcriptional TGP is exclusive to larva stages and does not
involve AS
Based on the results of our previous study, where TGP of heat tolerance
was exclusively detected in larvae, we tested whether the
transcriptional landscape of plasticity reflects our observation at the
phenotypic level (Diaz et al., 2020) . Our previous phenotype data
correlated with a higher level of parent-to-offspring environmental
predictability in the early stages, which is a hypothesis that has not
been formally tested using transcriptional data. As predicted, we found
substantial transcriptional responses associated with parental
acclimation in larvae, with 419 DE genes, while only one DE gene was
detected in adult samples (Figure 2a). On the other hand, no genes
responding through AS were detected with significant TGP in larvae
(Figure 2a).