Transcriptional TGP is exclusive to larva stages and does not involve AS
Based on the results of our previous study, where TGP of heat tolerance was exclusively detected in larvae, we tested whether the transcriptional landscape of plasticity reflects our observation at the phenotypic level (Diaz et al., 2020) . Our previous phenotype data correlated with a higher level of parent-to-offspring environmental predictability in the early stages, which is a hypothesis that has not been formally tested using transcriptional data. As predicted, we found substantial transcriptional responses associated with parental acclimation in larvae, with 419 DE genes, while only one DE gene was detected in adult samples (Figure 2a). On the other hand, no genes responding through AS were detected with significant TGP in larvae (Figure 2a).