Experimental design and RNA-Seq
Previous studies have demonstrated adaptive within- (WGP) and
transgenerational plasticity (TGP) of heat tolerance following
acclimation treatments at 36 °C in parents and offspring of D.
mojavensis (Diaz et al., 2020). Here, we investigate the underlying
transcriptional bases of these types of plasticity by using the same
experimental design, which allows us to address fundamental questions on
how selection drives adaptive WGP and TGP while simultaneously
considering larval and adult stages of D. mojavensis . Our
approach involves a full factorial design where parents and offspring
were exposed to either an acclimation temperature of 36 °C or a control
treatment at 25 °C (Figure 1). RNA-Seq libraries were sequenced from
larva and adult samples collected in the offspring generation, with
three biological replicates for each of the four combinations of
acclimation treatments (Figure 1).