RNA extraction, cDNA library construction, and sequencing
Total RNA was extracted using Direct-zol RNA kit (Zymo Research). Both
RNA quality and quantity were inspected on a Bioanalyzer (Applied
Biosystems/Ambion). cDNA libraries were created using KAPA Stranded
mRNA-Seq Kit according to the manufacturer’s instructions. The 24
RNA-seq libraries were sequenced at Novogene Inc. using the HiSeq SBS v4
High Output Kit on Illumina platform flow cells with runs of 2 x 150 bp
paired-end reads. Illumina’s HiSeq Control Software and CASAVA software
(Illumina, Inc.) were used for base calling and sample demultiplexing.