Functional analysis of DE genes associated with WGP and TGP
DE genes with transcriptional TGP are enriched by 11 GO categories, five of which are shared with WGP (Figure S3). Overlapping genes between WGP and TGP are significantly associated with 11 categories, six of them already present in the independent analyses (Figure S3). Thus, transcriptional TGP is associated with some GO categories found in WGP, such as i) proteolysis, in addition to ii) DNA replication, and ii) several additional structural/cell/metabolic functions (Figure S3). The overlapping genes between WGP and TGP are significantly linked to different pathways of structural development, such as i) apical construction, ii) embryonic cell shape, iii) body morphogenesis, and iv) cuticle development, as well as pathways of v) collagen production, vi) oxidoreductase, and vii) endopeptidase activity (Figure S3).
Figure 4. Transcriptional landscape comparing differential gene expression (DE) between WGP and TGP in the larva of D. mojavensis. All comparisons were performed between acclimated samples at 36 °C and control treatments at 36 °C using three biological replicates following FDR corrections. a) Scatterplot shows the transcriptional landscape with relative expression levels (log2-fold-change) for DE genes with significant WGP vs. TGP. b) Boxplots show the level of differential gene expression (log2-fold-change) for genes of the heat-shock response (i.e., proteolysis and Hsps) with significant WGP and TGP.