Figure 5. Relationship between DE genes with transcriptional within- (WGP) and transgenerational plasticity (TGP) in the larva ofD. mojavensis. All comparisons were performed between acclimated samples at 36 °C and control treatments at 36 °C using three biological replicates following FDR corrections. The Venn diagrams show a)the number of up and down-regulated genes with WGP vs. up-regulated genes with TGP; b) the number of up and down-regulated genes with WGP vs. down-regulated genes with TGP; and c) Boxplots showing the transcriptional trajectories of the overlapping DE genes significantly associated WGP and TGP, across the acclimation treatments performed in the parental and offspring generations. Significant comparisons (α < 0.05) following GLM analysis are indicated with *.