The heat-shock response and transcriptional WGP
The acclimation effect within a generation, commonly known as heat hardening or heat-shock response, has been widely investigated across several organisms for decades (Krebs, 1999; Krebs and Bettencourt, 1999; Hoffmann et al., 2003; Sgrò et al., 2010; Kellermann and Sgrò, 2018; Diaz et al., 2020). We found that DE genes are linked to functions that are typical of heat hardening, including the expression of proteolytic pathways, heat-shock proteins (HSPs), and other molecular functions that might protect tissues from the damage caused by high thermal exposures (Dahlgaard et al., 1998; Sørensen et al., 2005; Bahrndorff et al., 2010; Diaz et al., 2015; Mahat et al., 2016; Cai et al., 2017). In contrast, AS genes are involved in multiple structural, cell, and metabolic functions, including muscle assembly, as well as in different mechanisms of gene regulation, such as spliceosome activity, chromatin remodeling, and translation regulation.