Because IR changes are more likely linked to mechanisms of down-regulation by transcript degradation, we tested this hypothesis by estimating IR changes between acclimated samples at 36 °C vs 25 °C for WGP and TGP. We compared IR changes for significantly up- vs down-regulated DE genes. A GLM analysis was performed using categories of up- and down-regulation as independent variables and the level of IR change as the dependent variable for each comparison. GLM analysis was performed following square root transformation to normalize the error distribution and to achieve homoscedasticity.