Detection of platelet-specific antibodies
Peripheral blood was collected before the onset of treatment to detect
platelet-specific antibodies. Antibodies, anti-GPIIb/IIIa and
anti-GPIb/IX, were detected using a commercial kit(PAKAUTO; GTI
Diagnostics Inc, Waukesha, WI, USA). The solid-phase modified
antigen-capture enzyme-linked immunosorbent assay (ELISA) was employed
to detect platelet-specific antibodies 13. The ratio
of the patient’s antibody titer to the control’s antibody titer was
defined as the antibody titer ratio. We defined the ratio as positive
when the antibody titer ratio was >1 in plasma or eluent
and defined the ratio as negative when the antibody titer ratio was
<1 in both plasma and eluent.