2.1.1 Extraction steps for the reverse micellar extraction
Protein extraction using the reverse micelle system consists of two
steps: forward extraction and backward extraction. Forward extraction
involves the dissolution of the protein into the polar aqueous core
(Fig. 1 ). Subsequently, in the backward extraction, the
protein-containing solution is released from the polar aqueous core and
transferred to a fresh aqueous phase for recovery (Leser ME, 1989).