Fig 4 IgE-binding epitopes of fish parvalbumins. (A) Aligned protein sequences of fish parvalbumins showing major IgE-binding epitopes. Purple-shaded sequences are epitopes previously identified. Boxed sequences are IgE-binding regions identified in this study. (B) Signal intensity of the overlapping parvalbumin peptides of grouper compared between grass carp allergic and tolerant subjects. Signal intensity comparison between allergic and partial tolerant subjects of overlapping parvalbumin peptides of (C) salmon β-1, (D) salmon β-2, (E) cod, (F) grouper and (G) grass carp. Significant difference between allergic and partial tolerant individuals (*q-value <.05) on Mann-Whitney test are showed.