Appendix
Detailed protocol for preparing a 6 % (w/v) Alginate bioink
A 6% (w/v) alginate solution was prepared by dissolving alginic acid sodium salt in double distilled water (ddH2O) under continuous stirring on a hot plate. The alginate was prepared and used immediately before each experiment. A 0.3 M CaCl2solution was prepared and used for cross-linking of the alginate post-printing.