Thermal Settings:
Bed minimum and maximum temperature: both initial lines in the script were changed to 0 degrees Celsius. This was done as the hotends were removed from the bed platform during modification of the printer into a bioprinter.
#define BED_MINTEMP 5 set to #define BED_MINTEMP 0#define BED_MAXTEMP 120 set to #define BED_MAXTEMP 0
  1. Cold extrusion: The script was changed to allow cold extrusion. #define PREVENT_COLD_EXTRUSION set to // #define PREVENT_COLD_EXTRUSION
  2. Extruder minimum/maximum temperature: As with the bed platform temperature settings, both were changed to 0 as the extruder hotend and thermistor were removed during modification. #define EXTRUDE_MINTEMP 170 set to #define EXTRUDE_MINTEMP0
  3. Preheating bed and extruder: These temperatures were also set to 0 as there was no need for preheating, without hotends available. #define PREHEAT_1_TEMP_HOTEND 180 set to #define PREHEAT_1_TEMP_HOTEND 0#define PREHEAT_1_TEMP_BED 70 set to #define PREHEAT_1_TEMP_BED 0