Figure 4: Patterns of differential gene expression and protein abundance in the context of environmental sampling. Animals were sampled across the course of the day (lower x-axis) from the local depths (y-axis) experienced profound differences in food type/availability (fluorescence as a proxy for photosynthetic plankton density), temperature and oxygen (right panel, from CTD cast). Although copepods primarily experienced low light levels, they responded to the diel cycle (upper x-axis demarcates sunrise and sunset times) with migrations. These environmental and behavioral distinctions among sampling points resulted in distinct changes in the transcriptome (blue) and proteome (orange), suggestive of diel partitioning of repair, migratory effort, and metabolism that may have substantial consequences for biogeochemical and ecological signatures across the daily cycle.
Supplementary Files
1. Detailed transcriptomic, proteomic and bioinformatics methods
2. Transcriptome and differential expression
3. Detailed ‘omics results and figures
4. Transcriptome Go Enrichment results
5. Proteome and differential abundance