3.7 Enzyme assay
The activity of FPOX was evaluated. Results from this study shown that
rFPOX was an active enzyme. In FPOX activity, was used as coloring agent
for peroxidase substrate. The activity of our FPOX enzyme was compared
with FPOX enzyme from HbA1c Kit (SEKISUI, Japan). For blood samples from
diabetic patients, similar results were obtained but our results were
gained after 20 mins instead of 5 mins obtained by the kit.