3.7 Enzyme assay
The activity of FPOX was evaluated. Results from this study shown that rFPOX was an active enzyme. In FPOX activity, was used as coloring agent for peroxidase substrate. The activity of our FPOX enzyme was compared with FPOX enzyme from HbA1c Kit (SEKISUI, Japan). For blood samples from diabetic patients, similar results were obtained but our results were gained after 20 mins instead of 5 mins obtained by the kit.