3.4 The effect of arginine and sorbitol in culture medium on the formation of inclusion body
The effect of arginine and sorbitol on protein solubility was investigated in shuffle strain. Results show that nearly a similar amount of protein expression was observed in the presence of sorbitol compared to control (Fig.4). In the other side, in the presence of arginine in the culture media the expression of rFPOX was very low and concluded that arginine had the least effect on solubility compared to sorbitol. The concentration of total protein in the presence of sorbitol (2.43 mg/ml) was higher than arginine and control culture media (2.13 mg/ml).