2 Materials and Methods
The cDNA sequence of wild-type Eupenicillium terrenum FPOX
(Accession Number: AB116146) in the form of sub-clone in pET22b(+) was
synthesized by Generay Company (Hongkong). Horseradish peroxidase (HRP),
TMB (3,3’,5,5’-Tetramethylbenzidine) and protein marker were provided by
Applied Biotechnology Center (Iran, Kermanshah).
Isopropyl-β-D-thiogalactoside (IPTG) was purchased from Sigma (St.
Louis, MO, USA). Escherichia coli strains BL21 (DE3) and sHuffle
were obtained from IBRC (Tehran, Iran). Arginine, sorbitol, glycerol and
DMSO were purchased from Merck (Germany). All other chemicals and
reagents were of the highest commercial grade available.