3.1 Expression of FPOX
Recombinant FPOX, approximately 47 KDa, was mainly expressed as inclusion bodies in BL21 and shuffle strains under different conditions of IPTG and induction temperature (Fig.1) and no considerable soluble protein was observed in supernatant. The level of protein expression was same in both of strains. The different physicochemical characteristics were evaluated by protparam tool, and shown that, FPOX composed of 437 amino acids with a molecular weight of about 48 KDa and contained a theoretical pI of 6.68. This molecule consisted of 229 hydrophobic amino acids (52.4 %) and 208 hydrophilic residues (47.6 %) and, the acidic and basic amino acids were 51 (11.7 %) and 62 (14.2 %), respectively.