Variations based on microsatellites data
All the 16 microsatellite loci were polymorphic in C. bifida andC . micholitzii , and a total of 358 and 705 alleles were identified, respectively. The total number of alleles (N T) of each population varied from 74 (PAK) to 155 (SEK); the private alleles (N P) varied from 8 (KN) to 155 (SEK); the allelic richness (A R) varied from 3.032 (TA) to 6.477 (SEK); the mean number of alleles (N a) for each population ranged from 4.625 (PAK) to 9.688 (SEK) and the effective number of alleles (N e) varied from 53 (PAK) to 34 (SEK) (Table 2). The SEK population of C . micholitziihad the most N T, N p,A R, N a and N e (Table 2). The observed heterozygosity (H o) and expected heterozygosity (H E) were 0.291 (PAK) to 0.511 (SJX) and 0.420 (OC) to 0.705 (SEK), respectively (Table 2). The percentage of polymorphic loci (PPB ) had higher values that varied from 81.25% to 100% (Table 2).