Network of haplotypes at the cpDNA and each low copy nuclear
locus
In haplotype networks, haplotypes belonging to C. bifida were
represented in white, while haplotypes belonging to C.
micholitzii were represented in black (Figure 2). Based on cpDNA
data and the three nuclear genes (PHYP , AC5 andPPRC ), haplotypes of C. bifida can be clustered together
and haplotypes of C. micholitzii can be clustered together
without mixing, except the nuclear gene PHYP had one haplotype
(A5) shared by the two species. Due to the recombination, the haplotype
networks of AAT and SAMS showed reticulated structures. It
was difficult to identified ancestral haplotypes in C. bifida .
For C. micholitzii , haplotypes cpH11, A5, B17, C8 and C9, D10 and
E13 were located at the internal node of networks respectively and
appeared frequently, so they may be ancestral haplotypes. The remaining
haplotypes were linked to the central haplotype, which were deduced
newly derived haplotypes.