Laboratory Measurements
Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), estradiol (E2), Anti-Müllerian hormone (AMH), fasting insulin and fasting plasma glucose were determined, as previously reported19. To define insulin resistance, homeostasis model assessment (HOMA-IR) was made using the equation: fasting glucose (mmol/L) × fasting insulin (mIU/L)/22.5.