FIGURE LEGENDS
Figure 1. Sampling localities and
population genetic structure of the Aquilegia viridifloracomplex. A. Geographical distributions of the sampled A.
viridiflora complex, where colors in circles distinguish groups. The
genetic structure showed ADMIXTURE proportions of genetic clusters for
each individual of the four lineages at the best K value (K = 4), and
each bar represents an individual. The scale in the figure is
1:20,000,000, B. Population genetic structure inferred by ADMIXTURE when
K= 3-5, C. phylogenetic relationships of A. viridiflora complex
according to the maximum likelihood (ML) method, and blue, red, light
green and orange represent the NE, EL, CN and NW lineages, respectively.
The asterisk indicates that the bootstrap value is 100.
Figure 2. A. NeighborNet diagram
based on genome, B. nucleotide diversity (θπ) ofA. viridiflora complex.
Figure
3.
Demographic
history of Aquilegia viridiflora complex and population genetic
analysis. A. Results for the D-statistics. The solid red dot represents
that the absolute value of Z-scores is greater than 3, and the hollow
red dot represents that the absolute value of Z-scores is less than 3,
B. ancestral population sizes, population divergence times, and
migration rates were assessed by fastimcoal2, C. pairwise
FST and DXY between different groups, D.
LD decay of the four lineages of A. viridiflora complex. Except
that the difference between the two groups marked in the figure is not
significant, there is a significant difference between any other two
groups.
Figure 4. Genomic regions with
signals of selection and adaptation to local environmental conditions.
A. Bayescan results for genome-wide scans to detect outlier SNPs based
on locus-specific FST values. Red dots represent qval less than 0.05 and
indicated the SNPs under the positive selection, B. relationship of
genetic distance and geographical distance, C. outlier SNPs associated
with bioclimate factors. The red dots indicate that the SNP in the
sliding window was selected by the environment; gray dots represent
other regions in the genome. The map shows the spatial variation in
different bioclimate factors.