2.1. Preparation and extraction of aqueous extract
The desired plant was individually powdered by a home mill. Pour 50 gr
of each of the powders separately into a flask, add 400 ml of distilled
water to shake it in a shaker at 160 rpm for one day. The extract from
this step was filtered and added again to the remaining amounts of
distilled water and the previous step was repeated. The extracts
collected in these two stages were distilled in a vacuum distillation
apparatus at 45°C and 70 rpm until the remaining volume reached
one-fifth of the initial volume. After that, the contents of the tank
were transferred into the petri dish and dried completely in the oven at
50°C within a few days. Different dilutions were prepared from the
obtained extract with phosphate buffer saline and sterilized using 0.22µ
filters. Finally, the diluted extracts were stored in sterile microtubes
in the freezer until use.