2.1. Preparation and extraction of aqueous extract
The desired plant was individually powdered by a home mill. Pour 50 gr of each of the powders separately into a flask, add 400 ml of distilled water to shake it in a shaker at 160 rpm for one day. The extract from this step was filtered and added again to the remaining amounts of distilled water and the previous step was repeated. The extracts collected in these two stages were distilled in a vacuum distillation apparatus at 45°C and 70 rpm until the remaining volume reached one-fifth of the initial volume. After that, the contents of the tank were transferred into the petri dish and dried completely in the oven at 50°C within a few days. Different dilutions were prepared from the obtained extract with phosphate buffer saline and sterilized using 0.22µ filters. Finally, the diluted extracts were stored in sterile microtubes in the freezer until use.