UV-Vis analysis:
In this study, silver nanoparticles were formulated at a temperature of 80 °C and a duration of 30 minutes. The reaction solution color changed from dark red to black due to the interaction between the plant extract and the silver salt solution, and it is the first sign of producing silver nanoparticles, was quite evident. After that, the absorption spectrum of the nanoparticle solution using a spectrophotometer showed a peak wavelength of about 442 nm, which shows the silver nanoparticle presence (Figure 6). The above findings in the present study proved that the color change is a function of time and with the passage of time and the progress of the reaction, the concentration of silver nanoparticle increases. By measuring the optical density of the reaction solution in different time intervals, we came to the conclusion that the rise in the silver nanoparticle concentration causes the color of the reaction solution to change to black.