Figure 9. Breakthrough of XMuLV overlayed with a blue dextran run. Both datasets performed at 7.3cm/hr.
The pre breakthrough fraction contained 2.85x101 virus copies/mL indicating little to no breakthrough as expected. The expected breakthrough fraction contained 6.78x106 virus particles and the post breakthrough fraction contained 1.65x106 virus particles. This follows a similar profile to the blue dextran elution profile, and most importantly shows virus breakthrough does not occur earlier than blue dextran.
In addition to breakthrough characterization, virus inactivation was also evaluated at multiple residence times across the column. Virus inactivation was measured at residence times of 2, 10, 20, and 30 minutes. Log inactivation data is shown in Figure 10.