Figure 9.
Breakthrough of XMuLV overlayed with a blue dextran run. Both datasets
performed at 7.3cm/hr.
The pre breakthrough fraction contained 2.85x101 virus
copies/mL indicating little to no breakthrough as expected. The expected
breakthrough fraction contained 6.78x106 virus
particles and the post breakthrough fraction contained
1.65x106 virus particles. This follows a similar
profile to the blue dextran elution profile, and most importantly shows
virus breakthrough does not occur earlier than blue dextran.
In addition to breakthrough characterization, virus inactivation was
also evaluated at multiple residence times across the column. Virus
inactivation was measured at residence times of 2, 10, 20, and 30
minutes. Log inactivation data is shown in Figure 10.