Abstract
The complete mitochondrial genomes of two Prophantis species in
the tribe Trichaeini (Lepidoptera: Crambidae) were sequenced using
high-throughput sequencing technology. They were assembled and
annotated: the complete mitogenomes of P. octoguttalis andP. adusta were 15,197 bp and 15,714 bp, respectively, and contain
13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes,
and an A + T-rich region. Their arrangement was consistent with the
first sequenced mitogenome of Lepidoptera, from Bombyx mori(Bombycidae). The nucleotide composition was obviously AT-biased, and
all protein-coding genes, except for the cox1 gene (CGA), used
ATN as the start codon. Except for trnS1 , which lacked the DHU
arm, all tRNA genes could fold into the clover-leaf structure.
Phylogenetic trees of Crambidae were reconstructed based on mitogenomic
data using Maximum likelihood (ML) and Bayesian inference (BI) analysis
methods. Results showed that Trichaeini in this study robustly
constitute a monophyletic group in Spilomelinae, with the relationships
(Trichaeini + Nomophilini) + ((Spilomelini + (Hymeniini + Agroterini)) +
Margaroniini). However, the affinities of the six subfamilies
Acentropinae, Crambinae, Glaphyriinae, Odontiinae, Schoenobiinae and
Scopariinae within the “non-PS Clade” in Crambidae remained doubtful
with unstable topologies or low supports.