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\begin{document}

\title{Plant-based green-synthesized silver nanoparticles: Introducing a modern
chemotherapeutic drug to treat the human lung carcinoma}



\author[1]{Jingsheng Chen}%
\affil[1]{Ezhou Central Hospital}%


\vspace{-1em}



  
  \date{June 03, 2024}


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\begin{abstract}
In this study, Urtica dioica L extract as a stabilizing and reducing
agent was utilized to synthesize silver nanoparticles in the aqueous
medium. Various techniques containing UV-Vis. spectroscopy, FT-IR
spectroscopy, X-ray diffraction (XRD), scanning electron microscopy
(SEM), and Transmission electron microscopy (TEM) were used to
characterize the synthesized nanoparticles (AgNPs). On the other hand,
the MTT assay was run to evaluate anti lung cancer activity of AgNPs.
The uniform spherical morphology ranging from 26.22 to 65.18 nm was
detected in the SEM images for the biosynthesized nanoparticles. The
crystal size of AgNPs, according to the XRD analysis, was 48.11 nm. In
the cellular and molecular part of the recent study, the treated cells
with AgNPs@Urtica dioica L were assessed by MTT assay for 48 h about the
cytotoxicity and anti-human lung adenocarcinoma properties on normal
(HUVEC) and lung adenocarcinoma cell lines i.e. HLC-1, LC-2/ad, and
PC-14. The viability of malignant lung cell line reduced
dose-dependently in the presence of AgNPs@Urtica dioica L. The IC50 of
AgNPs@Urtica dioica L were 201, 108 and 143 \selectlanguage{greek}µ\selectlanguage{english}g/mL against HLC-1,
LC-2/ad, and PC-14 cell lines, respectively. In the antioxidant test,
the IC50 of AgNPs@Urtica dioica L and BHT against DPPH free radicals
were 125 and 60 \selectlanguage{greek}µ\selectlanguage{english}g/mL, respectively.%
\end{abstract}%



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\textbf{Hosted file}

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