Sholl Analysis
Sholl Analysis is a classical method to analyze neuronal complexity by drawing a series of concentric circles with the soma as the center. This is done by obtaining the number of intersections of dendrites that have distances at 10mm intervals from the soma. The number of intersections is used to reflect the complexity of the cell. We used Imaris software (version 7.7.2) for dendritic complexity sholl analysis. The steps for dendritic complexity sholl analysis are as follows: color inversion–select the soma as a center–select the dendritic end–perform semi-automatic dendritic analysis. Dendrite branching should be flipped to 3D to match the selection in order to distinguish real dendrite branches. When the drawing is finished, select specific values in the statistics tab - filament No. sholl intersections (Pillaiet al. , 2012).