2.3 Phylogenetic analysis
The VP4/VP2 region reference sequences of RV, consisting a set of 153
sequences, were obtained from the GenBank database and incorporated into
the analysis. For phylogenetic tree construction, MEGA X software was
employed, utilizing the neighbor-joining method. The robustness of the
phylogenetic trees was evaluated through bootstrap analysis with 1000
replicates. Genetic diversity, sequence identity, and amino acid
variation were analyzed using BioEdit software. The sequences identified
in this study have been submitted to the GenBank database, with
accession numbers ranging from OR575561 to OR575592.