2.3 Phylogenetic analysis
The VP4/VP2 region reference sequences of RV, consisting a set of 153 sequences, were obtained from the GenBank database and incorporated into the analysis. For phylogenetic tree construction, MEGA X software was employed, utilizing the neighbor-joining method. The robustness of the phylogenetic trees was evaluated through bootstrap analysis with 1000 replicates. Genetic diversity, sequence identity, and amino acid variation were analyzed using BioEdit software. The sequences identified in this study have been submitted to the GenBank database, with accession numbers ranging from OR575561 to OR575592.