FIGURE 4: The pelagic environment drives differences in expression between species and implicates cell cycle regulation as a mode of increased bone deposition. (A) Volcano plot depicting DE between species in the benthic environment (n=984 total; n=549 MZ in red; n=435 TRC in blue). (B) Nearly four times the number of DEGs are detected in the pelagic environment (n=3761 total; n=1927 MZ in red; n=1834 TRC in blue). (C) The venn diagram shows that not only are there more DEGs in the pelagic versus benthic environment, but that most (813/984 = 83%) of the DEGs detected in the benthic environment are also DE in the pelagic environment. Alternatively, only 22% (813/3761) DEGs detected in the pelagic environment are also DE in benthic fish. (D, E) Enriched GO terms are shown for MZ versus TRC in the pelagic (D) and benthic (E) environments. Colors are representative of the -log10(p-value). Gray bars indicate no significance. Gene counts are given as a total for both species along with the corresponding GO term. Many terms enriched for in MZ in both environments are associated with cell cycle (e.g., cell cycle, cell division, etc).