Figure 2. Characterization of droplets for cytoplasmic protein delivery. (A) The concentration of the released β-galactosidase from the droplets after incubation with various concentrations of reduced glutathione (GSH). (B) Dynamic light scattering intensity of the droplets after down-sizing using an extruder. (C) Confocal microscopic images of DLD1 cells treated with nano-droplets modified with anti-epiregulin antibody and (D) without anti-epiregulin antibody. The antibody was labeled with a fluorescent dye (Alexa Fluor 647: AF647) (pink). The nano-droplets were labeled with DPPE-PEG-TAMRA (orange). Scale bar: 10 μm.