Figure 1. Schematic illustration depicting cytosolic protein delivery using ultrasound-guided vaporization of phase-change nano-droplets. (A) The chemical structure of the protein anchoring reagent that anchors the protein onto the nano-droplets (DPPE-PEG-PyDTP) and the corresponding anchoring reaction. (B) Chemical structures of the nano-droplet modifiers for antibody modification (DPPE-PEG-Biotin), stabilization (DPPE-PEG-OMe), and labeling (DPPE-PEG-TAMRA). (C) Schematic of cellular uptake and ultrasound-guided intracellular vaporization of the droplets carrying cargo proteins for cytosolic delivery through endosomal escape.