perfluorocarbon nano-droplets
Satoshi Yamaguchi,1* Miho Kisaka,1Kotaro Higashi,1 Ayumu Ishijima,2Takashi Azuma, 3 Keiichi Nakagawa,2,5 Yoshikazu Shibasaki,4Ichiro Sakuma2,5,6 and Akimitsu Okamoto1*
1Department of Chemistry & Biotechnology, The University of Tokyo, Tokyo, Japan
2Department of Precision Engineering, The University of Tokyo, Tokyo, Japan
3Center for Disease Biology and Integrative Medicine, The University of Tokyo, Tokyo, Japan
4Research Center for Advanced Science and Technology (RCAST), The University of Tokyo, Tokyo, Japan
5Department of Bioengineering, The University of Tokyo, Tokyo, Japan
6Medical Device Development and Regulation Research Center, The University of Tokyo, Tokyo, Japan
Correspondence: Satoshi Yamaguchi and Akimitsu Okamoto, Department of Chemistry & Biotechnology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
E-mail : yamaguchi@chembio.t.u-tokyo.ac.jp or okamoto@chembio.t.u-tokyo.ac.jp
Keywords: protein delivery system, nano-droplets, ultrasound, mammalian cells, synthetic polymers
Abbreviations: PCND, phase-change nano-droplet;EREG, epiregulin; PEG, poly(ethylene glycol);DPPC, dipalmitoyl phosphatydylcholine; DPPE,dipalmitoyl phosphatydylethanolamine; PFH, perfluorohexane;β-Gal, β-galactosidase; FDG,fluorescein-di-(β-D-galactopyranoside); GSH, reduced glutathione; TAMRA, tetramethyl rhodamine; Sap,Saporin; PBS, phosphate-buffered saline; ME,2-mercaptoethanol; Ex, excitation wavelength; Em,emission wavelength; Biotin-NHS, Biotin N-succinimidyl ester;DMEM, Dulbecco’s modified Eagle’s medium.